dihydroethidium  (Servicebio Inc)

Journal: Poultry Science

Article Title: Targeted intestinal delivery of luteolin microcapsules as a precision nutritional strategy to alleviate heat stress and enhance growth performance in broilers

doi: 10.1016/j.psj.2026.106976

Figure Lengend Snippet: Effect of Lut on 42 °C -induced ROS accumulation in cells. (A) Effect of Lut (5, 10, 20, 40, and 100 μM) on cell viability, n = 6. (B) Protective effect of Lut (5, 10, 20, 40, and 100 μM) on 42 °C-induced cytotoxicity, n = 6. (C, D) Effect of Lut on 42 °C-induced ROS generation measured by 10 μM DCFH-DA fluorescent probe. The results were expressed as the mean DCF fluorescence intensity (n = 6). (E, F) Effect of Lut on 42 °C-induced superoxide anion radical generation measured by 10 μM DHE fluorescent probe. The results were expressed as the mean DHE fluorescence intensity (n = 6). The normal control group was incubated at 37 °C for 6 h. The HS group was incubated at 37 °C for 5 h and then at 42 °C for 1 h. Data are expressed as mean ± SD. * represents the significant difference compared with the control group ( P < 0.05), ** represents the significant difference compared with the control group ( P < 0.01). # represents the significant difference compared with the HS ( P < 0.05). ## represents the significant difference compared with the HS ( P < 0.01).

Article Snippet: 2′,7′-Dichlorodihydrofluorescein diacetate (DCFH-DA), dihydroethidium (DHE), cell counting kit-8 (CCK-8), RIPA lysis buffer, phenylmethanesulfonyl fluoride (PMSF), and BCA protein assay kit were purchased from Beyotime Biotech.

Techniques: Fluorescence, Control, Incubation

Journal: International Journal of Nanomedicine

Article Title: Application of Cerium-Tannic Acid-Formaldehyde Coordination Polymer Colloidal Nanomaterials to Alleviate Lipopolysaccharide-Induced Acute Lung Injury

doi: 10.2147/IJN.S604112

Figure Lengend Snippet: Antioxidant effect of Ce-TA on LPS-induced ALI mice. ( A ) Schematic illustration of the establishment and treatment schedule of LPS-induced ALI mice. ( B ) Quantitative analysis of the relative fluorescence intensity of DHE in lung tissues of mice in different groups. ( C ) Representative DHE fluorescence images of lung tissues of mice in different groups. ( D ) Concentration of MPO in BALF of mice in different groups. Concentrations of ( E ) SOD and ( F ) MDA in lung homogenates of mice in different groups. Data are presented as means ± SD (n = 5 per group). ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: Lung tissue sections were stained with 2 μg/mL of DAPI (G1012, Servicebio) and dihydroethidium (DHE, D7008, Servicebio) at 37 °C for 30 min. After three washes with PBS, sections were imaged using a fluorescence microscope (Axio Observer, ZEISS), and data were analyzed by ImageJ software.

Techniques: Fluorescence, Concentration Assay